WebMay 15, 2024 · Ggt1-Cre mice were provided by Eric Neilson, Northwestern University (Chicago, IL), and described previously . The Ggt1 promoter is predominantly expressed in the kidney proximal tubules, which was designed to generate the Cre-expressing mice, with undetectable expression in the liver and pancreas . WebFeb 6, 2024 · Homozygous CrATloxP female mice (C57BL/6J) were bred to male γ-glutamyl transferase Cre mice (Tg-(Ggt1-cre)M3Egn/J, mixed Balb/cJ/C57BL/6 background; The Jackson Laboratory). Offspring heterozygous to CrATloxP were backcrossed to the CrATloxP mice. This cross produced offspring of which 50% were PTC-specific CrAT …
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WebMar 19, 2024 · 基因敲除小鼠基础知识. 持续活化的Cre敲除基因的效率较高，但敲除基因的时间点不可控，导致基因敲除可能发生在发育早期或非预期的实验时间窗 。. 诱导型CreER虽具有时间可控性，提高了遗传操作的精确度，但敲除基因效率较低。. 为了提高诱导型CreER的 … Web010611 B6.129P2(Cg)- Ighg1 tm1(cre)Cgn /J This strain expresses Cre recombinase from the endogenous Ighg1 , immunoglobulin heavy constant gamma 1, locus. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in the study of gene function in germinal center, class switched memory and … is behr paint non toxic
《科学》：中国科学家首次实现高分辨成像观察小鼠胚胎发育全过 …
WebCre鼠常用于与Flox小鼠杂交进行条件性的目的基因敲除，但也有研究将Cre鼠用于切割位于报告基因前的终止序列，以达到荧光标记特定细胞的目的，进一步研究细胞命运。 Webline expressing Cre recombinase under the control of the Ggt1 promoter (28). As indicated in Figure 1A, to generate a renal prox-imal tubule–specific -KO mouse line (Pten ptKO), we crossed Pten this Ggt1-Cre mouse with Pten exon 5–floxed mice (Ptenfl/fl); exon 5 encodes the essential phosphatase core domain of the PTEN protein (27). Web004782 STOCK Tg(KRT14-cre)1Amc/J A congenic version of this strain is available as Stock No. 018964 . The K14-Cre transgene has a human keratin 14 promoter directing expression of Cre recombinase. These K14-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in the ectoderm and its derivatives (including the skin, … one hot mama charlotte nc